Morphometric Analysis

MBF Labs can provide you with detailed morphometric analysis

Complete morphometric analysis requires precise representation and feature extraction of the object of interest. MBF Labs has expert scientists who can provide you with expert consultation and analysis of objects of interest.

Neuron Reconstruction Services

MBF Labs can perform detailed morphometric analysis of neurons. This technique will create a visual representation of how individual or multiple neurons are positioned in three dimensional space. At MBF Labs, we use Neurolucida to reconstruct objects as large as whole organs or as small as dendritic spines. We can perform numerous analyses, including Sholl and polar histograms. When evaluating neurons, this program enables accurate tracing of the dendritic trees, axonal arborization, and the soma. This reconstruction technology can also be expanded to other areas of interest, including representation of an injury from the image data of an MRI.

Data from reconstructed neurons contained in single or serial sections include:

  • 3-D measurements of bifurcating and trifurcating branching processes
  • Dendritic spine distribution and density
  • Synaptic bouton distribution and density
  • Branch complexity
  • Volumes of branches and somas
  • Surface area
  • Cytoarchetectonic organization
  • Morphometric analyses

A 3-D visualization can be created from the reconstruction and provided as an .xml file appropriate for use in presentations, documents, digital reproductions and supplementary materials.

Vessel Reconstruction Services

Using the most advanced vessel tracing software, Vesselucida 360, MBF Labs’ experts are able to reconstruct microvasculature in 3D. This provides reliable data on vasculature-specific metrics such as segments and nodes counts, frequency of anastomoses, vessel surface and volume, and more.

MBF Labs is able to quantify changes in vasculature due to traumatic brain injury or stroke, changes in blood vessels of the retina due to diabetes,  and new blood vessels due to a cancerous tumor.