MBF Labs :  A contract research organization specializing in stereology services, neuron and vessel reconstruction, and slide imaging.

Stereology Services: Unbiased Quantitative Analyses of Tissue Specimens

We realize that there is a growing need for researchers to greatly increase the rate of their data collection. By outsourcing your unbiased stereology data collection and analysis to us, we better enable you to devote your valuable time for experimental design and interpretation. We provide leading stereology services for university, pharmaceutical, biotechnology, and government labs worldwide.

As a trusted source of stereological analysis, our service is ideal for anyone who is looking for expert stereological analysis but lacks in-house instrumentation. We can handle management of all types of studies, from small pilot studies to full-scale experimental analyses. Our scientists will collect the data, interpret the results, and prepare a comprehensive report for you. Whether it is brain, lung, or other biological tissue, you will always get the accurate results that you need.

Neuron Reconstruction Services

MBF Labs can perform detailed morphometric analysis of neurons. This technique will create a visual representation of how individual or multiple neurons are positioned in three dimensional space. We offer services from full neuron tracing to localized dendritic spine analysis.

Neuron reconstruction is the most accurate method to quantify neuronal morphology. MBF Labs’ expert scientists provide full consultation and analysis of the neuron, using the most advanced neuron tracing software, Neurolucida 360. Reconstructions can be completed using your provided slides or images. Comprehensive metrics from reconstructed neurons include:

  • Branch order and distance from soma, including 3D Sholl analysis
  • Soma, dendritic and axonal arbor volumes and surface areas
  • Dendritic spine distribution and density
  • Varicosity distribution and density

Vessel Reconstruction Services

Using the most advanced vessel tracing software, Vesselucida 360, MBF Labs’ experts are able to reconstruct microvasculature in 3D. This provides reliable data about the length, connections and complexity of microvessels. MBF Labs is able to quantify changes in vasculature due to traumatic brain injury or stroke, changes in blood vessels of the retina due to diabetes, and new blood vessels due to a cancerous tumor.

Slide Scanning and Imaging

Slide Scanning is the method of choice for complete, high-resolution, two and three-dimensional digital representations of microscope specimen slides. We use this technology to create a montage composed of hundreds or thousands of individual images obtained from multiple fields-of-view at the desired magnification using either brightfield or fluorescent microscopy. The final image montage provides a large field- of-view combined with  the detail and resolution from the high magnification. This provides visualizing histology at any desired magnification, the ability to share tissue and analysis results with colleagues worldwide without time delay, and digital archiving, which prevents data loss caused by the fading of sensitive stains. Our Slide Scanning services can be used as a valuable complement to our stereology or morphology services or as a standalone project.

Image Volume Generation from Serial Section Images

Using our software, BrainMaker and TissueMaker, MBF Labs can create 3D volumes of the brain or other entire organs from serially sectioned 2D images. This can assist with cell mapping, cytoarchitectonics and other areas to create a comprehensive anatomical reference. The resulting volume will provide the ability to easily see the location of all cells expressing a particular gene with full anatomical context.

Morphometric Analyses

In addition to providing extensive morphometric analyses on neuron reconstructions, we can provide analyses on serial section reconstructions of specific areas of interest and entire brain maps. This reconstruction technology can also be expanded to other areas of interest, including making a 3D serial section representation of an injury from the image data.

Bring Expert Analysis to your Research with MBF Labs

Our staff scientists are skilled at all aspects of data collection and management. We are adept at projects ranging from small exploratory pilot studies to large, complex projects. MBF Labs uses the most advanced systems available to collect and analyze your data. Our skillful technicians are experienced and efficient. When you outsource your stereology analyses to MBF Labs, you get reliable results quickly. No other contract research organization offers the breadth of service, high competency and level professionalism that MBF Labs provides.

An additional benefit in allowing us to collect the data for your study is the elimination of the necessity for you to invest in costly specialized equipment, personnel and facilities. We will apply our experience as the recognized leaders in neuroscience quantification to help you effectively attain your research goals.

MBF Labs will be there to guide you every step of the way from designing the study, collecting the data, evaluating the results and providing a formal written report of the analyses. In the final report you will not only get the results from the data collection, you will also receive an interpretation of the results along with statistical analyses.

Let us help you

Let MBF Labs be an instrumental part of your research by providing you with the most expert and dependable stereology services, data collection and analyses.

You can watch a short introduction to MBF Labs here: